GENERAL LABORATORY SKILLS

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• Pipette, weigh, sterilize, filtrate, titrate, wash/clean glassware, prepare media, practice sterile techniques, light microscopy, dissection (vertebrae and invertebrate), bacteria culture. Proficient. 

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BIOCHEMISTRY SKILLS

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• Product Purification 

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       -  Thin-layer chromatography (TLC), 400+ times: I used TLC in various mixtures of different solvents

          (including hexane, cyclohexane, benzene, diethyl ether, dichloromethane, ethyl acetate, acetone,

          acetonitrile, ethanol, methanol) to ensure reaction completion. Example: completion of a Fischer

          Esterification on tyrosine. 

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       -  Column chromatography, 30+ times: I used column chromatography when polarity differences

          between products and byproducts are minimal or when TLC reveals unexpected results. I have

          experience determining proper solvent (same solvents as listed above) mixtures and using solvent

          gradients based on TLC results. Example: separation of esterified L-phenylalanine from L-

          phenylalanine. 

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       -  Liquid-liquid extractions, ~40 times: I used separatory funnels with organic and aqueous solvents to

          isolate desired products. Example: extraction of nitrophenyl acetate from a byproduct of acetic

          anhydride using liquid-liquid extraction then rotary evaporation with 97% yield. 

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       -  Recrystallization, 10+ times: Example: obtained desired products in nitrophenol synthesis with 95%   

          yield. 

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       -  Centrifugation, sparingly: for crude separation of cell components. Example: centrifugation of cultivated

          non-pathogenic E. Coli to isolate cytosol.

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• Molecular Characterization 

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               -  Nuclear Magnetic Resonance (NMR), 60MHz spectrometer, 40+ runs: for accurate molecular

                  characterization. I prepped NMR samples and read the results. Example: characterization of synthesized

                  novel b-lactam antibiotics.

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               -  Fourier Transform Infrared Spectroscopy (FTIR), 50+ runs: for product identification when changes 

                  in bonding interactions are significant. Example: confirmation of successful imine condensation - loss of

                  aldehyde and formation of signature C-N imine double bond.

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• Enzyme Kinetics 

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               -  Beer’s law, Michaelis-Menton, and Lineweavur-Burk plots, 30+ graphs: for studying trypsin hydrolysis

                  rates in solvents of different polarities. ​

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• UV-VIS Spectrophotometry

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               -  UV-VIS spectrophotometry, 40+ times: for research on colorimetric substrates of serine proteases such

                  as nattokinase. I used a 3000 UV-VIS spectrophotometer to quantify the absorption wavelengths of the

                  substrates and the hydrolyzed products. I tracked the differences in peak absorption wavelengths over

                  time to quantify the respective enzyme hydrolysis rates. ​

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• In Silico Study 

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-  Density-Functional Theory Calculations (DFT), Orca program package, 200+ calculations: for               compound structure optimizations in preparation for docking experiments. Example: DFT calculations       on a library of in silico SARS-CoV-2 Main Protease inhibitors.

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-  Molecular Docking, Autodock Vina and Swissdock, 150+ docking experiments: for analysis of the

   interactions and relative binding affinities of different ligands with respective proteins. Example:                 analysis of relative binding affinities between the SARS-CoV-2 Main Protease and a library of                     inhibitors. Utilized Chimera for molecular visualization.

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-  Time-Dependent Density-Functional Theory (TDDFT), 80+ runs: determined the effects of side             chain modifications on excitation energy of 4 solvatochromic compounds by looking at the energy             differences between their highest occupied molecular orbitals (HOMO) and lowest unoccupied                   molecular orbitals (LUMO).

-  Molecular Dynamics (MD) Simulations, GROMACS package, 10+ simulations: determined                     predictive compound stability of precise and tunable inorganic nanoparticle composite arrangements           within soft matrix materials.   

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CLINICAL SKILLS

 

Mentee, level-1 trauma center, 5+ clinical research seminars, Medical Immersion Summer Academy, Highland Hospital and Samuel Merritt University, Oakland, CA, July 2019, 40 hours/week

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• Certificates 

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               -  Cardiopulmonary Resuscitation (CPR), EMS Safety, 2019 - Present. 

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               -  Bloodborne Pathogen, EMS Safety, 2019 - Present.

 

• Medical Diagnostic/Laboratory Techniques

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               -  Blood tests, biopsy, EKG, ultrasound, and vitals, 10+ practices.

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• Surgery Skills

 

             -  Practiced splinting and suturing using pig skin.​

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BIOTECHNOLOGY SKILLS

 

• DNA sequencing, Polymerase Chain Reaction, Polyacrylamide/DNA Gel, Western Blotting, Northern Blotting and Southern Blotting, and Electrophoresis. Learned in theory.      

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STATISTICAL ANALYSIS SKILLS

  

• Student's t-test, contingency tables, chi-square and z tests, analysis of variance, univariate linear regression modeling, graphing categorical and quantitative data. Fluent.  

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• Multivariate regression modeling, experimental and observational study designs, sampling and simulation. Novice.

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